- ¥780 - 2200
- 康朗生物
- kl-1535R
- 中国/美国/德国
- 2025年07月10日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
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- 详细信息
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-1535R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
Alkaline Phosphatase, Tissue Non-Specific isozyme antibody
- 抗体名：
组织非特异性碱性磷酸酶抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from Alkaline Phosphatase, Tissue Non-Specific isozyme:52-150/524
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
50ul 100ul 200ul
Alkaline Phosphatase, Tissue Non-Specific isozyme antibody
| 中文名称 | 组织非特异性碱性磷酸酶抗体 |
| 别 名 | AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. 碱性磷酸酶，组织非特异性同工酶； |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 56kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Alkaline Phosphatase, Tissue Non-Specific isozyme:52-150/524 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Function: This isozyme may play a role in skeletal mineralization. Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Post-translational modifications: Glycosylated. DISEASE: Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510]. Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500]. Similarity: Belongs to the alkaline phosphatase family. SWISS: P09242 Gene ID: 249 Database links: Entrez Gene: 249 Human Entrez Gene: 11647 Mouse Entrez Gene: 25586 Rat Omim: 171760 Human SwissProt: P05186 Human SwissProt: P09242 Mouse SwissProt: P08289 Rat Unigene: 75431 Human Unigene: 288186 Mouse Unigene: 82764 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ALP广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织,孕妇、骨折愈合期、骨软化症。佝偻病、骨细胞癌、骨质疏松、肝脓肿、肝结核、肝硬变、白血病、甲状腺机能亢进时，血清碱性磷酸酶亦可升高. |
| 产品图片 |  Sample: Placenta (Mouse) Lysate at 30 ug Primary: Anti- Alkaline Phosphatase Tissue Non-Specific isozyme (bs-1535R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 60 kD  Blank control (blue line): Hep G2(blue). Primary Antibody (green line): Rabbit Anti-ALPL antibody (bs-1535R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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Alkaline Phosphatase, Tissue Non-Specific isozyme antibody
¥780 - 2200
