- ￥1700 - 4000
- GeneTex
- 美国
- GTX100519
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100519
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay
- 浓度：
0.083 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HMGA2
- 抗体英文名：
HMGA2 antibody - ChIP grade
- 抗体名：
HMGA2 抗体 - ChIP grade
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HMGA2 antibody (GTX100519) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HMGA2 antibody (GTX100519) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human HuCCT1 xenograft .
HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody (GTX100519).
Western blot analysis was performed using HMGA2 antibody (GTX100519) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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