- ￥6100
- GeneTex
- 美国
- GTX30614
- 2025年07月16日
- WB, ICC/IF, FACS, IP
- Mouse
- Human, Mouse, Rat, Mustelid, Primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Mustelid, Primate
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX30614
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
EP300
- 抗体英文名：
EP300 antibody [RW128]
- 抗体名：
EP300 抗体 [RW128]
- 规格：
100 μg
ICC/IF analysis of PFA-fixed HeLa cells using GTX30614 EP300 antibody [RW128].
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.05% Triton X-100 in PBS
Dilution : 5 μg/mL
WB analysis of HeLa nuclear extract using GTX30614 EP300 antibody [RW128].
Dilution : 1:250
FACS (Intracellular staining) analysis of THP-1 cells using GTX30614 EP300 antibody [RW128].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
with non-immune reactivity for immunoglobulin G. J. Immunol. 111:1401-1406. Richman, D. D., Cleveland, P. H., Oxman, M. N., and Johnson, K. M. 1982. The binding of staphylococcl protein A by the sera of different animal species. J. Immunol. 128
Silver Enhancement of Colloidal Gold
. for 1-nm gold, for Nanogold 4 to 5 min (20 - 22°C). The final pH is 4 (see Burry 1995, p. 220). Remove blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate
Immunoflourescence of Cultured Cells
Materials L-lysine coated glass cover slips or charged glass slides Neutral Buffered Formalin (Sigma HT50-128) 0.5% NP40 in PBS (2.5 N HCl or 0.07 N NaOH for BrDU staining only) Primary and secondary
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