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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Mustelid, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX30618
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, IP, ChIP assay, EM, in vitro
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This is specific for p300 protein. This recognizes residues 1921-2023.
- 抗体英文名:
EP300 antibody [RW105]
- 抗体名:
EP300 抗体 [RW105]
- 规格:
100 μg
FACS (Intracellular staining) analysis of THP-1 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
ICC/IF analysis of PFA-fixed HeLa cells using GTX30618 EP300 antibody [RW105].
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.05% Triton X-100 in PBS
Dilution : 5 μg/mL
FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
WB analysis of HeLa nuclear extract using GTX30618 EP300 antibody [RW105].
Dilution : 1:250
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文献和实验Su KW et al., Cells 2022 (PMID:36231010)
Monoclonal Antibody Production Protocol
of debris, rounded and refractive under phase contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
predictions to be made about their structure and function that can then be tested experimentally. The starting point for all of this is the ability to isolate the specific cDNA encoding the protein of interest from libraries that contain on the order of 105 –
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
predictions to be made about their structure and function that can then be tested experimentally. The starting point for all of this is the ability to isolate the specific cDNA encoding the protein of interest from libraries that contain on the order of 105 –
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