- ￥6100
- GeneTex
- 美国
- GTX27045
- 2025年07月01日
- WB, ICC/IF, IHC-P, FACS, MS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX27045
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, MS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
We do not recommend use of this product for Mouse.
- 抗体英文名：
MGMT antibody [MT 23.2]
- 抗体名：
MGMT 抗体 [MT 23.2]
- 规格：
100 μl
ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Blue : Hoechst 33258
Dilution : 1:50
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:2000
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:1000
FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100
FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
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文献和实验Mojas N et al., Genes Dev 2007 (PMID:18079180)
Khalil S et al., Oncotarget 2016 (PMID:27057640)
【操作步骤】6.2 分文章作者，亲手教你如何轻松搞定生信文章
大家好，今天跟大家分享一篇文章「DDX60 is associated with glioma malignancy and serve as a potential immunotherapy biomarker」。 该文章是由笔者于 2021 年 2 月发表于《Frontiers in Oncology》杂志上的，目前该杂志中科院分区 2 区，影响因子 6.2 分。 今天就通过该文章跟大家分享一下肿瘤方向生信文章的套路及绘图方法～ 信息发展到这个年代，许多科研道友仍然闻「代码」色变，总想走
Notes: The key variable in MT spindown experiments is ATP. Under high ATP conditions,conventional MAPs are selectively co-sedimented with microtubules. In theabsence of ATP (or in presence of AMPPNP which induces rigor binding
MT Spindowns from Extracts Arshad Desai Notes: The key variable in MT spindown experiments is ATP. Under high ATP conditions
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