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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX27045
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, MS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
We do not recommend use of this product for Mouse.
- 抗体英文名:
MGMT antibody [MT 23.2]
- 抗体名:
MGMT 抗体 [MT 23.2]
- 规格:
100 μl
ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Blue : Hoechst 33258
Dilution : 1:50
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:2000
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:1000
FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100
FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
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文献和实验Mojas N et al., Genes Dev 2007 (PMID:18079180)
Khalil S et al., Oncotarget 2016 (PMID:27057640)
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