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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human p53. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX118730
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
p53
- 抗体英文名:
p53 antibody [C2C3], C-term
- 抗体名:
p53 抗体 [C2C3], C-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
p53 antibody immunoprecipitates p53 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of p53 protein by 2.5 μg of p53 antibody (GTX118730) 12% SDS-PAGE The immunoprecipitated p53 protein was detected by p53 antibody (GTX118730) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 24hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
10% SDS PAGE
GTX118730 diluted at 1:1000
p53 antibody [C2C3], C-term detects p53 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
p53 antibody [C2C3], C-term (GTX118730) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验人总P53蛋白(Total P53)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人总P53 蛋白 ( Total P53 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 P53 单抗包被于酶标板上,标准品和样品中的 P53与单抗结合,加入生物素化的抗人 P53 ,形成免疫复合物连接在板上,辣根
豚鼠总P53蛋白(Total P53)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠总P53 蛋白 ( Total P53 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 P53 单抗包被于酶标板上,标准品和样品中的 P53与单抗结合,加入生物素化的抗豚鼠 P53 ,形成免疫复合物连接在板上,辣根
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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