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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human RPS6 that contains serines 235 and 236. The sequence is conserved in mouse and rat.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX12864
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.
- 抗体英文名:
RPS6 (phospho Ser235/236) antibody
- 抗体名:
RPS6 (phospho Ser235/236) 抗体
- 规格:
50 μl
WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB analysis of whole cell extracts (20 μg lysate) of HeLa (Lane 1), treated for 20 minutes with 200 nM of PMA (Lane 2), A549 (lane 3) serum starved A549 (lane 4) and serum starved for overnight followed by Serum Released (lane 5) using GTX12864 RPS6 (phospho Ser235/236) antibody.
Dilution : 1:500
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文献和实验Goodson WH 3rd et al., Carcinogenesis 2011 (PMID:21890461)
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