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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant GST (pGEX), Schistosoma japonicum
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX19585
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IP, ELISA
- 浓度:
0.10 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Specifically interacts with GST of Schistosoma japonicum that is encoded by the pGEX expression vectors.Does not interact with human, mouse, rat mammalian GST proteins.
- 抗体英文名:
GST tag antibody [BDI340]
- 抗体名:
GST tag 抗体 [BDI340]
- 规格:
50 μg
WB analysis of GST-PIN1 recombinant protein using GTX19585 GST antibody [BDI340] at 0.5μg/ml. Lane 1: 100ng ; Lane 2: 50ng ; Lane 3: 25ng ; Lane 4: 10ng ; Lane 5: 5ng ; Lane 6: 2ng ; Lane 7: 1ng
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文献和实验先介绍一下His Tag,为5-15 His 串在一起的小肽, 在表达纯化中用的较为普遍的一种Tag, 从本质上说它是一种亲和性Tag,但也个案证明其也能做一些兼职工作:增加蛋白可溶性,提高蛋白稳定性。但它的本质工作是与IMAC亲和,使纯化过程变的更加程序化,更具可控性。 相对与其他亲和Tag,如 GST, Flag, Strep, 它的特点可谓鲜明: 一,小,浓缩的都是精华,6个His 放在一起约2.5KD大小。His 的等电点为7.6,在常用的纯化缓冲体系(pH 8.0)中
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
鉴于原核表达的两大热门系统,his tag系统和gst系统,从园子里归纳出以下帖子,以方便大家参考。 这是关于his tag的讨论贴 蛋白纯化高手请指点(his) http://www.dxy.cn/bbs/thread/276543 请问哪位做过6-His蛋白? http://www.dxy.cn/bbs/thread/36760 his融合表达问题!再问载体! http://www.dxy.cn/bbs/thread/633019 his-tag标签纯化
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