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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Aspartoacylase. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX113389
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度:
1.33 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Aspartoacylase
- 抗体英文名:
Aspartoacylase antibody [N1C3-2]
- 抗体名:
Aspartoacylase 抗体 [N1C3-2]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Aspartoacylase(GTX113389) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Aspartoacylase antibody [N1C3-2] detects Aspartoacylase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Aspartoacylase protein stained by Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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文献和实验Choi BR et al., Cell Rep 2020 (PMID:32375055)
Larson VA et al., Elife 2018 (PMID:29596047)
Orthmann-Murphy J et al., Elife 2020 (PMID:32459173)
Huber K et al., Biochim Biophys Acta Mol Cell Res 2019 (PMID:30595160)
Xin W et al., Cell Rep 2019 (PMID:31116973)
KatharinaHuber et al., Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2018 (Epub)
Lee NJ et al., Brain 2018 (PMID:29688408)
Gautier EL et al., J Exp Med 2014 (PMID:25024137)
Long PM et al., J Biol Chem 2013 (PMID:23884408)
Sommer A et al., Gene 2012 (PMID:22750302)
Xing YL et al., Cell Rep Methods 2023 (PMID:36936074)
Chen Y et al., bioRxiv 2023 (PMID:36747743)
Leah DeFlitch et al., Front Cell Neurosci 2022 (PMID:35465615)
Yanan Chen et al., Elife 2021 (PMID:33752802)
Lucas A et al., Front Neurosci 2021 (PMID:34858133)
Cardona HJ et al., Brain Behav 2021 (PMID:34480532)
Nair G et al., Neuroimage 2020 (PMID:32828923)
i 的概率,任何时刻一个变量被选取是随机的)的二项分布。这一二项分布可以很好地近似为参数泊松分布,而且计算起来也更容易。 7. 见注 2。对于这种情况,λ 可以计算如下。不同于等概率情况,λi 在这里是不同的。首先,为了计算 λ,我们需要计算所有的 n = 2110 个参数 λi,这个工作量很大,但是这里包含了很多重复,这一特征可以用来简化计算。而且,让我们确定一些由五元组(n1,n2,n3,n4,n5)确定的十肽,由密码子的数量可知,它们的概率在所描述的十肽中分别为 1/64,2/64,3/64
an important role in regulation of the immune system. In the method described, hepatoma MH134 (Fas− and FasL− ) is transfected with murine Fas and FasL cDNA. A single administration of monoclonal anti-Fas antibody efficiently suppresses the growth of F6b (MH134
SDS-PAGE,蛋白转印,western Blot试剂配制
1.5 mol/L Tris (PH8.8)1.称量181.7g Tris置于1L烧杯中。2.加入约800 ml去离子水,充分搅拌溶解。3.用浓盐酸调节pH值至8.8。4.定容至1L.5.高压灭菌后,室温保存注意:应使溶液冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差异很大,温度每升高1oC,溶液的pH值大约降低0.03个单位。(参照kara公司Catalog-N1)1 mol/L Tris (PH6.8)1.称量121.1g Tris置于1L烧杯中。2.加入约800 ml
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