- ￥1700 - 4000
- GeneTex
- 美国
- GTX113115
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX113115
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Actinin 4
- 抗体英文名：
alpha Actinin 4 antibody [N2C1], Internal
- 抗体名：
alpha Actinin 4 抗体 [N2C1], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [N2C1], Internal (GTX113115) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.
Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HepG2 (GTX27900)
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Ilaria Grossi et al., Int J Mol Med 2021 (PMID:33416118)
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane 3) Block overnight @4C in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 (important if you are re-using
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