Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Chicken
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX112898
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
p21 Cip1
抗体英文名:
p21 Cip1 antibody
抗体名:
p21 Cip1 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Sample (30 ug of whole cell lysate) A: HCT116 B: HCT116 treated with 30uM cisplatin for 24hr 12% SDS PAGE GTX112898 diluted at 1:1000
p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1xenograft. p21 Cip1 antibody (GTX112898) dilution: 1:500.
p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.p21 Cip1 stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.Antigen Retrieval: EDTA buffer, 20 min
p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% PFA at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX112898) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application Reference
Abdalla BA et al., Front Genet 2020 (PMID:32158470)
Lin JH et al., Stem Cells International 2019 2019()
Cai B et al., Cell Death Dis 2018 (PMID:29511169)
Lee WY et al., Oncotarget 2015 (PMID:26363315)
Ma G et al., Int J Biol Sci 2014 (PMID:24644428)
Ma M et al., Cell Commun Signal 2023 (PMID:36635672)
Abdalla BA et al., Life Sci 2021 (PMID:33290791)
Abdalla BA et al., Front Physiol 2018 (PMID:30333755)