![Bid antibody [N1C3-2]](https://img1.dxycdn.com/2023/0711/255/2344116916935858761-14.jpg)
- ￥1700 - 4000
- GeneTex
- 美国
- GTX110568
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length human Bid Recombinant protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX110568
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377403)
- 抗体英文名：
Bid antibody [N1C3-2]
- 抗体名：
Bid 抗体 [N1C3-2]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/227/4836393559807287771.jpg)
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/875/6587316800907287771.jpg)
Bid antibody [N1C3-2] detects Bid protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg H1299 whole cell lysate/extract
D. 30 μg HeLa whole cell lysate/extract
E. 30 μg HepG2 whole cell lysate/extract
F. 30 μg Molt-4 whole cell lysate/extract
G. 30 μg Raji whole cell lysate/extract
7.5 % SDS-PAGE
Bid antibody [N1C3-2] (GTX110568) dilution: 1:1000
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/768/6564753791907287771.jpg)
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid(GTX110568) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/746/4456995420907287771.jpg)
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] (GTX110568) or Bid antibody [N1C3] (GTX101323).
Western blot analysis was performed using Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/152/2994673812907287771.jpg)
Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% PFA at RT for 15 min.
Green: Bid protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/158/6739017010907287771.jpg)
Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% PFA/culture medium at 37ºC for 30 min.
Green: BID protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
Blue: Hoechst 33343 staining.
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/643/8659856599807287771.jpg)
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.
![Bid antibody [N1C3-2]](https://img1.dxycdn.com/p/s14/2024/0410/954/0677914129807287771.jpg)
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
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文献和实验Ovadje P et al., Oncotarget 2016 (PMID:27564258)
Carpentieri A et al., Cell Death Dis 2015 (PMID:26561783)
Pu YF et al., Int J Clin Exp Pathol 2014 (PMID:24817952)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
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