- ￥1700 - 4000
- GeneTex
- 美国
- GTX109935
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP, PLA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Ku80. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX109935
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, PLA
- 浓度：
0.41 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Ku80
- 抗体英文名：
Ku80 antibody [N3C2], Internal
- 抗体名：
Ku80 抗体 [N3C2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 μg of Ku80 antibody (GTX109935) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (GTX109935) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Ku80 antibody [N3C2], Internal detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Ku80 stained by Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(GTX109935) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Bottoni G et al., Nat Commun 2019 (PMID:31467287)
Chen MK et al., J Biol Chem 2019 (PMID:30962283)
Chang CF et al., Cell Death Dis 2015 (PMID:25855964)
You BJ et al., Food Chem Toxicol 2014 (PMID:24373828)
Masuda Y et al., Biochem Biophys Res Commun 2011 (PMID:22037577)
Chiang JH et al., Hum Exp Toxicol 2013 (PMID:24064905)
Chen RY et al., Animals (Basel) 2020 (PMID:32630437)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
. For most substrates, it is believed, though it has been demonstrated experimentally only for a few, that the first ubiquitin moiety is conjugated, via its C-terminal Gly76 residue, to an ε-NH2 group of an internal Lys residue. Recent findings indicate
Using MZEF to Find Internal Coding Exons
) was designed to help identify one of the most important classes of exons, i.e. the internal coding exons, in human genomic DNA sequences. It is neither for predicting intronless genes, nor for assembling predicted exons into complete gene models
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