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- Bechman Coulter-AB Sciex
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- 608074
- 2025年07月10日
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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
until the solution becomes viscous and slightly clear. Do not allow the lysis reaction to proceed for more than 5 min. Add 350 μl Buffer N3 and invert the tube immediately but gently 4�6 times. To avoid localized precipitation, mix the solution gently
Primer Design（Manual and Automated Primer Design for SEQ and PHY Gaps）
. GC Clamp Avoid runs of identical nucleotides (e.g.ATCGACCCCCTAGAC). Similar Tm (+/-2℃) for primers in same reaction set. Unique to the template. Designing Primers for Sequencing Gaps
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