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文献和实验Figure 4.33.5 Gel electrophoresis analysis of hairpin loops (H; purified) and the click reaction mixture (C) on a 20% polyacrylamide/7 M urea gel 3 hr at a constant power of 20 W, using 0.09 M TBE buffer, pH
The Polymerase Chain Reaction (PCR)
• Template DNA • Reaction buffer (Tris, ammonium ions(and/or potassium ions), magnesium ions,bovine serum albumin) • Nucleotides (dNTPs) • Primers • DNA polymerase (usually Taq) PCR In Detail • Denature, anneal, extend and repeat the cycle 30 to 35
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
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