- ￥200 - 400
- NEB
- 美国
- #E1200L
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
Cas9 Nuclease NLS, S. pyogenes
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes sitespecific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases
from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein.
Product Source
An E. coli strain that carries the cloned Cas9 gene from Streptococcus pyogenes with a C-terminal Simian virus 40 (SV40) nuclear localization signal (NLS) and a N-terminal 6XHis tag.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Cas9 Nuclease Reaction Buffer | -20 | 10X |
Description
Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes sitespecific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases
from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein.
Product Source
An E. coli strain that carries the cloned Cas9 gene from Streptococcus pyogenes with a C-terminal Simian virus 40 (SV40) nuclear localization signal (NLS) and a N-terminal 6XHis tag.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Cas9 Nuclease Reaction Buffer | -20 | 10X |
Properties and Usage
Reaction Conditions
1X Cas9 Nuclease Reaction Buffer
Incubate at 37°C
1X Cas9 Nuclease Reaction Buffer:
20 mM HEPES
100 mM NaCl
5 mM MgCl2
0.1 mM EDTA
pH 6.5 @ 25°C
Storage Conditions
300 mM NaCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.4 @ 25°C
Notes
1. 1,000 nM is equal to 160 ng/μl.
References
1. Jinek M. et al. (2012). Science. 816-821. DOI: 10.1126/Science.1225829. Epub 2012 Jun 28, PubMedID: 22745249
南京赛泓瑞生物科技有限公司是专业从事生化试剂、分子生物学试剂、实验耗材及实验仪器研发、销售、服务为一体的专业性生物科技公司。本着服务科学的理念，赛泓瑞建立了生命科学研究工具的研发、生产、销售及技术服务平台，现已有数万余种产品，并每年不断开发新产品，为广大的生命科学研究人员、医学、药学、农业、环保等的科研人员提供科研工具性产品及技术服务.
经营范围：抑制剂、ELISA试剂盒、原代细胞和细胞株、化学试剂、蛋白、抗体、分子试剂和试剂盒。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Isolation of RNA from Difficult Tissues
Isolation of RNA from Difficult Tissues The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
A practical method for the preparation of total DNA from filamentous fungi
A practical method for the preparation of total DNA from filamentous fungi M.I. Borges, M.O. Azevedo, R. Bonatelli, Jr., M.S.S. Felipe and S. Astolfi-Filho Departamento de Biologia Celular, Universidade de Brasilia, 70910 Brasilia
技术资料暂无技术资料 索取技术资料
