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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
Cas9 Nuclease NLS, S. pyogenes
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes sitespecific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases
from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein.
Product Source
An E. coli strain that carries the cloned Cas9 gene from Streptococcus pyogenes with a C-terminal Simian virus 40 (SV40) nuclear localization signal (NLS) and a N-terminal 6XHis tag.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Cas9 Nuclease Reaction Buffer | -20 | 10X |
Description
Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes sitespecific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases
from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein.
Product Source
An E. coli strain that carries the cloned Cas9 gene from Streptococcus pyogenes with a C-terminal Simian virus 40 (SV40) nuclear localization signal (NLS) and a N-terminal 6XHis tag.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Cas9 Nuclease Reaction Buffer | -20 | 10X |
Properties and Usage
Reaction Conditions
1X Cas9 Nuclease Reaction Buffer
Incubate at 37°C
1X Cas9 Nuclease Reaction Buffer:
20 mM HEPES
100 mM NaCl
5 mM MgCl2
0.1 mM EDTA
pH 6.5 @ 25°C
Storage Conditions
300 mM NaCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.4 @ 25°C
Notes
1. 1,000 nM is equal to 160 ng/μl.
References
1. Jinek M. et al. (2012). Science. 816-821. DOI: 10.1126/Science.1225829. Epub 2012 Jun 28, PubMedID: 22745249
上海淳麦生物科技有限公司专注于感受态细胞、质粒、载体、农杆菌、酵母等生命科学领域的产品研发和技术服务支持。
上海淳麦生物科技有限公司在感受态细胞的研发方面，实力雄厚，拥有了克隆、表达、酵母和农杆菌4大类，共计百余种感受态细胞。在电击感受态方面，拥有了大肠杆菌和农杆菌电击感受态种类，极大提高了客户在转基因以及文库构建方面的成功率。
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