BbsI-HF®                     

Product Source

An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story)

Reagents Supplied

The following reagents are supplied with this product: 

	Store at (°C)	Concentration
CutSmart® Buffer	-20	10X
Gel Loading Dye, Purple (6X)	25	6X

 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X CutSmart® Buffer
Incubate at 37°C

1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C

Activity in NEBuffers

NEBuffer 1.1: 10%
NEBuffer 2.1: 10%
NEBuffer 3.1: 10%
CutSmart® Buffer: 100%

Diluent Compatibility

• Diluent B

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive 

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)
• BbsI
• Gel Loading Dye, Purple (6X)

Materials Sold Separately

• CutSmart® Buffer

1.  BbsI-HF has the same specificity as BbsI (NEB #R0539), but it has been engineered for reduced star activity. 

2.  Star activity may result from a glycerol concentration of > 5%.

3.  BbsI is an isoschizomer of BbvII.