Matrices listed below were spiked with certain level of HDAC2/YAF1/HD2/histone deacetylase 2/RPD3/transcriptional regulator homolog RPD3/YAF1/YY1-associated factor 1 and the recovery rates were calculated by comparing the measured value to the expected amount of HDAC2/YAF1/HD2/histone deacetylase 2/RPD3/transcriptional regulator homolog RPD3/YAF1/YY1-associated factor 1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
95
EDTA plasma(n=5)
86-101
91
heparin plasma(n=5)
93-99
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDAC2/YAF1/HD2/histone deacetylase 2/RPD3/transcriptional regulator homolog RPD3/YAF1/YY1-associated factor 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.