Matrices listed below were spiked with certain level of TNFRSF5/CD40 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF5/CD40 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
96
EDTA plasma(n=5)
95-105
99
heparin plasma(n=5)
86-102
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF5/CD40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.