- ￥5300
- GeneTex
- 美国
- GTX19389
- 2025年07月12日
- WB, ICC/IF, IHC-P, FACS, IP, ELISA
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016)
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX19389
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Her3 / ErbB3
- 抗体英文名：
Her3 / ErbB3 antibody [RTJ2]
- 抗体名：
Her3 / ErbB3 抗体 [RTJ2]
- 规格：
200 μg
FACS analysis of Hela cells using GTX19389 Her3 / ErbB3 antibody [RTJ2] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% PFA
ICC/IF analysis of MCF-7 cells using GTX19389 Her3 / ErbB3 antibody [RTJ2]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
FACS analysis of MCF-7 cells using GTX19389 Her3 / ErbB3 antibody [RTJ2] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% PFA
FACS analysis of Neuro-2a cells using GTX19389 Her3 / ErbB3 antibody [RTJ2] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% PFA
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文献和实验，EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体，而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体：hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本：蜡包埋人类肺部肿瘤抗原修复缓冲液：柠檬酸盐缓冲液（左）或者 EDTA 缓冲液（右）煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后，才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然，一些研究人员也使用水浴锅，但是，因为该步骤中所选择的装置对染色存在积极或消极的影响
number of genes (including ERBB2 , ERBB3 , VEGF , and EPO 5-8 ). However, rational design has not always yielded functional regulators in vivo , mainly because knowledge of both regulatory areas and of endogenous factors affecting transcription factor�DNA
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
plates containing SD solid medium lacking tryptophan (SD−Trp; see recipe ) Bait‐specific antibody or mouse monoclonal antibody against LexA (Dualsystems Biotech, see Internet Resources
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