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- 详细信息
- 技术资料
- 免疫原:
Purified recombinant PPAR alpha protein.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Rabbit, Bovine
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX12412
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PPAR alpha
- 抗体英文名:
PPAR alpha antibody [3B6/PPAR]
- 抗体名:
PPAR alpha 抗体 [3B6/PPAR]
- 规格:
50 μg
FACS analysis of Hela cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR].
Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)
FACS analysis of 3T3 cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
FACS analysis of Jurkat cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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