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TFIIE beta antibody

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月16日
  • WB, IP, ChIP assay
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human TFIIE beta. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX105029

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, IP, ChIP assay

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      TFIIE beta

    • 抗体英文名

      TFIIE beta antibody

    • 抗体名

      TFIIE beta 抗体

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Sample (30 ug of whole cell lysate)
    A: Jurkat nucleus
    10% SDS PAGE
    GTX105029 diluted at 1:5000

    产品细节图片2

    Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

    产品细节图片3

    TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 μg of TFIIE beta antibody (GTX105029) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (GTX105029) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
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    • Antibody cleanup

      in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta-mercaptoethanol to 0.1, glusulase

    图标技术资料

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    文献支持
    TFIIE beta antibody
    ¥1700 - 4000