- ￥2300
- GeneTex
- 美国
- GTX42055
- 2025年07月11日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, EM
- Mouse
- Human, Cynomolgus monkey, Rhesus Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human endothelial cell membrane vesicles.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Cynomolgus monkey, Rhesus Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX42055
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, EM
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes the human CD34. Clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion.
- 抗体英文名：
CD34 antibody [QBEnd/10]
- 抗体名：
CD34 抗体 [QBEnd/10]
- 规格：
25 μg
IHC-P analysis of placenta tissue using GTX42055 CD34 Class II antibody [QBEND/10].
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文献和实验Pilling D et al., J Leukoc Biol 2006 (PMID:16543402)
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
, and incubate for 1 hour on ice. Pellet cells, giving an antibody supernatant. Add this to a cell pellet prepared as in step 9. Be very careful not to get supernatants mixed up! Repeat steps 9 + 10 seven times, or as often as necessary to decrease
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