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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant (Human).
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX18656
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA, Neutralizing/Inhibition
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognises both recombinant and native IL8. It does not cross react with Monocyte Chemotactic Activating Factor (MCAF)or Regulated on Activation, Normal T-cell Expressed, and Secreted (RANTES).
- 抗体英文名:
CXCL8 / IL8 antibody [I8-60]
- 抗体名:
CXCL8 / IL8 抗体 [I8-60]
- 规格:
250 μg
Western blot analysis of Human IL-8 using clone I8-60. Concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of human IL-8.
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文献和实验标本。 2. 加20ul 1 N HC I ,盖紧,上下混匀。 2 - 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧,上下混匀。 4. 即用,或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 ( 注意:不同的标本E2F1 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本
for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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