- ￥7000
- GeneTex
- 美国
- GTX30221
- 2025年07月13日
- WB, ICC/IF, IHC
- Rabbit
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX30221
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
GluR1 (phospho Ser845)
- 抗体英文名：
GluR1 (phospho Ser845) antibody
- 抗体名：
GluR1 (phospho Ser845) 抗体
- 规格：
150 μl
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody (GTX30221). The immunolabeling is completely eliminated by treatment with λ-Ptase.
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文献和实验1 at S845,a site also phosphorylated by PKA. Activation of cGKII by cGMP increases the surface expression of AMPARs at extrasynaptic sites. Inhibition of cGKII activity blocks the surface increase of GluR1 during chemLTP and reduces LTP in the hippocampal
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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