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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
NogoA antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA.The immunogen is located within the first 50 amino acids of NogoA.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX85448
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.
- 抗体英文名:
Nogo A antibody
- 抗体名:
Nogo A 抗体
- 规格:
100 μg
WB analysis of mouse brain tissue lysate using GTX85448 Nogo A antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
IHC-P analysis of mouse brain tissue using GTX85448 Nogo A antibody.
Working concentration : 20 μg/ml
IHC-P analysis of mouse brain tissue using GTX85448 Nogo A antibody.
Working concentration : 2.5 μg/ml
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文献和实验阳性,未加一抗的呈阴性。染色结果显示成熟的少突胶质细胞突起丰富,互相形成蜘蛛网状(图3)。苏木素复染,异质细胞较少,90%以上为阳性细胞(图4)。 3 讨论 抑制神经再生基因Nogo的发现为视神经损伤的修复、视功能保护研究带来了希望。视神经胶质细胞包括星形胶质细胞和少突胶质细胞2种类型,分化不同,功能各异。成熟的少突胶质细胞不再具有分裂增殖能力,为分裂终期细胞[2],培养较为困难。1980年McCarthy[3]等建立了从脑灰质组织分离、纯化星形胶质细胞和少突胶质细胞的分离培养模型。目前国内外多采用
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