Matrices listed below were spiked with certain level of TRACP-5b and the recovery rates were calculated by comparing the measured value to the expected amount of TRACP-5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
94
EDTA plasma(n=5)
88-104
97
heparin plasma(n=5)
88-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRACP-5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.