Matrices listed below were spiked with certain level of ACTN3 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTN3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
95
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
85-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.