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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full-length protein expressed in 293T cell transfected with human SSB expression vector
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Dog, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83558
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, IP
- 浓度:
2.8 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SSB
- 抗体英文名:
SSB antibody [1E11]
- 抗体名:
SSB 抗体 [1E11]
- 规格:
100 μl
IP analysis of DDDDK tagged SSB overexpressed HEK293T lysate using GTX83558 SSB antibody [1E11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
ICC/IF analysis of HepG2 cells using GTX83558 SSB antibody [1E11].
WB analysis of various cell lines using GTX83558 SSB antibody [1E11].
Loading : 35 ug per lane
FACS analysis of Jurkat cells using GTX83558 SSB antibody [1E11].
Red : Primary antibody
Blue : Negative control antibody
WB analysis of HEK293T cells transfected with SSB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83558 SSB antibody [1E11].
Loading : 5 ug per lane
WB analysis of various samples using GTX83558 SSB antibody [1E11].
Loading : 10 ug per lane
Dilution : 1:200
WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83558 SSB antibody [1E11].
Loading : 10 ug per lane
Dilution : 1:1000
ICC/IF analysis of COS7 cells transiently transfected with SSB plasmid using GTX83558 SSB antibody [1E11].
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文献和实验Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo
in the intraembryonic aorta-gonad-mesonephros region at embryonic day (E) 10.5. After E11, these HSCs are thought to seed the liver and then the bone marrow just before birth. So far, many questions concerning the origin, properties, and functionality of these HSCs
Wholemount in situ hybridisation
. Each batch should ideally be tested. As a rough guide, use 5 min for E7.5, 7 min for E8.5, 9 min for E9.5, 11 min for E10.5, 12-14 min for E11.5. 8 Wash twice with PBTX for 5 minutes each. Be careful - the embryos are fragile
Figure 20.4.4 Observed binding enthalpy change from ITC depends on the buffer proton‐ionization enthalpy whenever binding is coupled to uptake or release of protons. Data are for a monoclonal antibody (MAb) protein–antigen (CD4) interaction (Doyle et al
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