- ￥1700 - 4000
- GeneTex
- 美国
- GTX101786
- 2025年07月15日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101786
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
hnRNP K
- 抗体英文名：
hnRNP K antibody
- 抗体名：
hnRNP K 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
hnRNP K antibody (GTX101786) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
hnRNP K antibody detects hnRNP K protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using hnRNP K(GTX101786) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody (GTX101786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
hnRNP K antibody detects hnRNP K protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
hnRNP K antibody (GTX101786) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
hnRNP K antibody (GTX101786) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101786 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
Matthew G Thompson et al., Elife 2020 (PMID:33269701)
Ke Zhang et al., Sci Adv 2021 (PMID:33547084)
Chou HC et al., Life Sci 2018 (PMID:29883720)
Generation of Antibody Molecules Through Antibody Engineering
Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite
to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection
Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature
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