- ￥7300
- GeneTex
- 美国
- GTX83583
- 2025年07月16日
- WB, IP
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full-length protein expressed in 293T cell transfected with human SORD expression vector
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX83583
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IP
- 浓度：
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Sorbitol Dehydrogenase
- 抗体英文名：
Sorbitol Dehydrogenase antibody [2A10]
- 抗体名：
Sorbitol Dehydrogenase 抗体 [2A10]
- 规格：
100 μl
WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83583 Sorbitol Dehydrogenase antibody [2A10].
Loading : 5 ug per lane
IP analysis of DDDDK tagged Sorbitol Dehydrogenase overexpressed HEK293T lysate using GTX83583 Sorbitol Dehydrogenase antibody [2A10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
WB analysis of various cell lines using GTX83583 Sorbitol Dehydrogenase antibody [2A10].
Loading : 35 ug per lane
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文献和实验Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement
In order to improve therapeutic antibodies efficacy in cancer patients, several strategies were developed. One of these strategies consists in the enhancement of effector functions. Antibody-dependent cellular cytotoxicity (ADCC) was shown
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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