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- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83612
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS
- 浓度:
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SMAD2
- 抗体英文名:
SMAD2 antibody [2C10]
- 抗体名:
SMAD2 抗体 [2C10]
- 规格:
100 μl
WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83612 SMAD2 antibody [2C10].
Loading : 5 ug per lane
FACS analysis of Jurkat cells using GTX83612 SMAD2 antibody [2C10].
Red : Primary antibody
Blue : Negative control antibody
ICC/IF analysis of COS7 cells transiently transfected with SMAD2 plasmid using GTX83612 SMAD2 antibody [2C10].
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
pH 7.2, 140 mM NaCl) containing 0.1% Tween 20 and 5% non-fat milk. The levels of phosphorylated (Ser465/467) and total Smad2, and expression of vimentin, a characteristic mesenchymal marker, were analyzed with specific primary antibodies (Cell
【热门靶点解析】TGF-β信号通路+重要作用+药物研发!(建议收藏)
反应,分为TGF-β1/2/3和BMP两条分支,分别激活Smad2/3和Smad1/5/8。TGF-β配体首先结合Ⅱ型受体(TβRⅡ),招募Ⅰ型受体(TβRⅠ/ALK5)形成复合物,TβRⅡ磷酸化激活TβRⅠ,后者进一步磷酸化Smad2/3。活化的R-Smad与Smad4结合,转入细胞核,与转录因子共同调控靶基因表达,介导细胞分化、凋亡、上皮-间质转化及免疫调节等生物学功能。该过程受Ⅲ型受体(TβRⅢ)辅助调控,确保信号精确传递。 TGF-β1的重要作用 1.TGF-β1定向诱导T细胞的分化 TGF
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