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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Dog, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83666
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IHC-P, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha 1 Antitrypsin
- 抗体英文名:
alpha 1 Antitrypsin antibody [9A1]
- 抗体名:
alpha 1 Antitrypsin 抗体 [9A1]
- 规格:
100 μl
IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human liver tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 5 ug per lane
IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83666 alpha 1 Antitrypsin antibody [9A1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
IHC-P analysis of human liver carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of various cell lines using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 35 ug per lane
WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Loading : 10 ug per lane
Dilution : 1:200
IHC-P analysis of human pancreas tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
FACS analysis of HEK293T cells transfected with either alpha 1 Antitrypsin plasmid(Red) or empty vector control plasmid(Blue) using GTX83666 alpha 1 Antitrypsin antibody [9A1].
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文献和实验He N et al., JCI Insight 2022 (PMID:35104244)
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