SEK1 / MKK4 antibody [8A8]

SEK1 / MKK4 antibody [8A8]

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  • ¥7300
  • GeneTex已认证
  • 美国
  • GTX84162
  • 2025年07月10日
  • WB, ICC/IF, IHC-P, FACS, IP
  • Mouse
  • Human, Dog
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    • 详细信息
    • 技术资料
    • 免疫原

      Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

    • 亚型

      IgG2b

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Dog

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX84162

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IHC-P, FACS, IP

    • 浓度

      2.36 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      SEK1 / MKK4

    • 抗体英文名

      SEK1 / MKK4 antibody [8A8]

    • 抗体名

      SEK1 / MKK4 抗体 [8A8]

    • 规格

      100 μl

    SEK1 / MKK4 antibody [8A8]

    FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84162 SEK1 / MKK4 antibody [8A8].

    SEK1 / MKK4 antibody [8A8]

    IHC-P analysis of human thyroid tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
    Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

    SEK1 / MKK4 antibody [8A8]

    IHC-P analysis of human kidney carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
    Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

    SEK1 / MKK4 antibody [8A8]

    IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84162 SEK1 / MKK4 antibody [8A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
    IP reaction : 2μg antibody / 500μl cell lysate

    SEK1 / MKK4 antibody [8A8]

    WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8].
    Loading : 10 ug per lane
    Dilution : 1:500

    SEK1 / MKK4 antibody [8A8]

    WB analysis of various cell lines using GTX84162 SEK1 / MKK4 antibody [8A8].
    Loading : 35 ug per lane

    SEK1 / MKK4 antibody [8A8]

    IHC-P analysis of human lung tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
    Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

    SEK1 / MKK4 antibody [8A8]

    IHC-P analysis of human thyroid carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
    Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

    SEK1 / MKK4 antibody [8A8]

    WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84162 SEK1 / MKK4 antibody [8A8].
    Loading : 5 ug per lane

    SEK1 / MKK4 antibody [8A8]

    IHC-P analysis of human pancreas tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
    Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

    SEK1 / MKK4 antibody [8A8]

    ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84162 SEK1 / MKK4 antibody [8A8].

    SEK1 / MKK4 antibody [8A8]

    ICC/IF analysis of HepG2 cells using GTX84162 SEK1 / MKK4 antibody [8A8].

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