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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human thymoma cell line HPB-ALL.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX21898
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
- 抗体英文名:
TCR V beta 5 antibody [MEM-262]
- 抗体名:
TCR V beta 5 抗体 [MEM-262]
- 规格:
100 μg
FACS analysis of human peripheral blood using GTX21898 TCR V beta 5 antibody [MEM-262].
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
consists of 5% (w/v) dried skim milk in Trisbufferedsaline/Tween-20 (TBS-T) buffer (50 mM Tris-HCl,pH 7.5, 150 mM NaCl, and 0.05% (v/v) Tween-20). 4. All antibodies are diluted 1:1,000 in 1% (w/v) dried skim milkin TBS-T, except the CRMP2 antibody (1:250
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