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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Cultured bone marrow cells
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX39794
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
- 抗体英文名:
Ly-6B.2 antibody [7/4]
- 抗体名:
Ly-6B.2 抗体 [7/4]
- 规格:
100 μg
IHC-Fr analysis of mouse lymph node tissue using GTX397943 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
IHC-Fr analysis of mouse lymph node tissue using GTX397943 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.
FACS analysis of mouse peripheral blood granulocytes using GTX39794 Ly-6B.2 antibody [7/4].
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
IHC-Fr analysis of mouse lymph node tissue using GTX39794 Ly-6B.2 antibody [7/4].
IHC-Fr analysis of C57BL/6 mouse spleen tissue using GTX39794 Ly-6B.2 antibody [7/4].
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文献和实验Shu F et al., Front Immunol 2018 (PMID:29896195)
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
、 62.5 、 31.2 、 0 pg /ml 为横坐标, OD 值为纵坐标,在坐标纸上作图,画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 E2F1 含量 ,再乘上稀释倍数即可 。 试剂盒性能 1. 灵敏度 : 最小的E2F1 检测浓度小于 15 pg/ml。 2. 特异性: 可同时检测重组或天然的人 E2F1 。 不与 人其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于8.9%
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