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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Rat T cell blasts.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX76383
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-Fr, FACS
- 浓度:
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD44
- 抗体英文名:
CD44 antibody [OX-50] (Azide free)
- 抗体名:
CD44 抗体 [OX-50] (Azide free)
- 规格:
500 μg
FACS analysis of rat spleen cells using GTX76383 CD44 antibody [OX-50] (Azide free).
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文献和实验Wu PT et al., J Biol Chem 2019 (PMID:31732563)
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
Blue Regeneration Buffer 2 M guanidine HCl or 1.5 M Na thiocyanate HiTrap Storage Buffer 10 mM Tris 7.5 1 ml 1M Tris 7.5 0.1 mM EDTA 20 ul 500 mM EDTA 50 mM NaCl 1 ml 5M NaCl 0.1% azide 0.1 g Na azide up to 100
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
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