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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
MLR generated rat T helper lymphocytes.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX75110
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
ICC/IF, IHC-Fr, FACS, IP
- 浓度:
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD4
- 抗体英文名:
CD4 antibody [OX-35] (Azide free)
- 抗体名:
CD4 抗体 [OX-35] (Azide free)
- 规格:
500 μg
IHC-Fr analysis of rat lymph node tissue using GTX75110 CD4 antibody [OX-35] (Azide free).
IHC-Fr staining of rat lymph node using Mouse anti Rat CD4 antibody (GTX75110), red in A and Mouse anti Rat CD8, green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power
IHC-Fr analysis of rat lymph node tissue using GTX75110 CD4 antibody [OX-35] (Azide free).
IHC-Fr analysis of rat lymph node tissue using GTX75110 CD4 antibody [OX-35] (Azide free).
IHC-Fr staining of rat lymph node using Mouse anti Rat CD4 antibody (GTX75110), red in A and Mouse anti Rat CD8, green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power
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文献和实验Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
Th17 Polarization of Mouse CD4 Cells
;g/ml). Incubate at 37 °C for 2 hours or 4 °C over night. A septically decant antibody solution from the plate. Wash plate 3 times with sterile PBS. Discard liquid. 2) Plate CD4 cells at 1x106/ml. Culture cells for 3 days in presence
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