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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
Amylin antibody [R10/99]
- 抗体名:
Amylin antibody [R10/99]
- 规格:
125μl
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
(for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend in 40 ml R10 media. Spin again and resuspend in 50 x the original cell
R10 media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells
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