- ￥6100
- GeneTex
- 美国
- GTX11022
- 2025年07月12日
- ICC/IF, IHC-P, IHC-Fr
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A peptide CATQRLANFLV corresponding to residues 40-50 of human amylin coupled to tuberculin
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX11022
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, IHC-P, IHC-Fr
- 靶点：
This antibody recognizes the islet amyloid polypeptide (amylin).
- 抗体英文名：
Amylin antibody [R10/99]
- 抗体名：
Amylin 抗体 [R10/99]
- 规格：
1 ml
IHC-P analysis of human pancreas tissue using GTX11022 Amylin antibody [R10/99].
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文献和实验Yoshimura M et al., Bioconjug Chem 2016 (PMID:27219875)
Zheng Z et al., J Clin Invest 2019 (PMID:31305260)
Hiroyuki Watanabe et al., Mol. Pharmaceutics 2018;15(12)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
(for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend in 40 ml R10 media. Spin again and resuspend in 50 x the original cell
R10 media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells
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