- ￥4300
- GeneTex
- 美国
- GTX70489
- 2025年07月11日
- WB, ELISA
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX70489
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 浓度：
1.36 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
The antibody works in any species where the TetR system is being used to regulate gene expression in mammalian cells.
- 抗体英文名：
Tet Repressor antibody
- 抗体名：
Tet Repressor 抗体
- 规格：
100 μl
Detection of Tet- Repressor (TetR) using anti-TetR (GTX70489) at a 1:1000 dilution.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody (GTX131984) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Indirect ELISA analysis performed by coating plate with recombinant Tet Repressor protein (162.21-2.53 nM). Coated protein was probed with Tet Repressor antibody (GTX70489) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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文献和实验Fang M et al., Cell 2010 (PMID:21074248)
Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
of the tet system, depends on the expression of the repressor (tetR ; Fuchs et al . 2002). By using tetop for one site, and lacop for the second, the risk of spurious pairing is eliminated. Useful pairs of GFP derivatives are CFP and YFP, or GFP and the new
Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
of the tet system, depends on the expression of the repressor (tetR ; Fuchs et al . 2002). By using tetop for one site, and lacop for the second, the risk of spurious pairing is eliminated. Useful pairs of GFP derivatives are CFP and YFP, or GFP and the new
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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