- ￥4300
- GeneTex
- 美国
- GTX70489
- 2025年07月16日
- WB, ELISA
- Rabbit
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX70489
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 浓度：
1.36 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
The antibody works in any species where the TetR system is being used to regulate gene expression in mammalian cells.
- 抗体英文名：
Tet Repressor antibody
- 抗体名：
Tet Repressor 抗体
- 规格：
100 μl
Detection of Tet- Repressor (TetR) using anti-TetR (GTX70489) at a 1:1000 dilution.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody (GTX131984) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Indirect ELISA analysis performed by coating plate with recombinant Tet Repressor protein (162.21-2.53 nM). Coated protein was probed with Tet Repressor antibody (GTX70489) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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- 作者
- 内容
- 询问日期
文献和实验Fang M et al., Cell 2010 (PMID:21074248)
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