相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
GST protein of the parasitic helminth Schistosoma japonicum expressed in E. coli
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX70195
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GST tag
- 抗体英文名:
GST tag antibody [3G10]
- 抗体名:
GST tag 抗体 [3G10]
- 规格:
100 μl
GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. Non-induced cell lysate from E. coli expressing GST-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins
10 % SDS-PAGE
GST tag antibody [3G10] (GTX70195) dilution: 1:10000
GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. 2 μg His-recombinant protein
B. 2 μg GST-recombinant protein
C. 1 μg GST-recombinant protein
D. 0.5 μg GST-recombinant protein
10 % SDS-PAGE
GST tag antibody [3G10] (GTX70195) dilution: 1:5000
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Luque CM et al., J Biol Chem 2003 (PMID:12427749)
Siepe D et al., Nat Cell Biol 2009 (PMID:19597489)
Takeharu Sakamoto et al., Commun Biol 2021 (PMID:34621018)
Morohoshi K et al., J Reprod Immunol 2021 (PMID:34700103)
Afolayan AJ et al., J Biol Chem 2016 (PMID:28028182)
Koppen-Rung P et al., J Virol 2016 (PMID:27053556)
先介绍一下His Tag,为5-15 His 串在一起的小肽, 在表达纯化中用的较为普遍的一种Tag, 从本质上说它是一种亲和性Tag,但也个案证明其也能做一些兼职工作:增加蛋白可溶性,提高蛋白稳定性。但它的本质工作是与IMAC亲和,使纯化过程变的更加程序化,更具可控性。 相对与其他亲和Tag,如 GST, Flag, Strep, 它的特点可谓鲜明: 一,小,浓缩的都是精华,6个His 放在一起约2.5KD大小。His 的等电点为7.6,在常用的纯化缓冲体系(pH 8.0)中
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
鉴于原核表达的两大热门系统,his tag系统和gst系统,从园子里归纳出以下帖子,以方便大家参考。 这是关于his tag的讨论贴 蛋白纯化高手请指点(his) http://www.dxy.cn/bbs/thread/276543 请问哪位做过6-His蛋白? http://www.dxy.cn/bbs/thread/36760 his融合表达问题!再问载体! http://www.dxy.cn/bbs/thread/633019 his-tag标签纯化
技术资料暂无技术资料 索取技术资料







