- ￥5600
- GeneTex
- 美国
- GTX17943
- 2025年07月16日
- WB, ICC/IF, FACS, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX17943
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS, ChIP assay
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
IKK alpha (phospho Ser176/Ser180)
- 抗体英文名：
IKK alpha (phospho Ser176/Ser180) antibody
- 抗体名：
IKK alpha (phospho Ser176/Ser180) 抗体
- 规格：
50 μl
WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody.
The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4)
Dilution : 1:500
ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-8 gene and the inactive SAT2 used as negative control target.
ICC/IF analysis of HeLa cells treated for 20 minutes with 50ng/ml of TNF-Alpha using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
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