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- 详细信息
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX17943
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, FACS, ChIP assay
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
IKK alpha (phospho Ser176/Ser180)
- 抗体英文名:
IKK alpha (phospho Ser176/Ser180) antibody
- 抗体名:
IKK alpha (phospho Ser176/Ser180) 抗体
- 规格:
50 μl
WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody.
The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4)
Dilution : 1:500
ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-8 gene and the inactive SAT2 used as negative control target.
ICC/IF analysis of HeLa cells treated for 20 minutes with 50ng/ml of TNF-Alpha using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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