IKK alpha (phospho Ser176/Ser180) antibody

IKK alpha (phospho Ser176/Ser1

80) antibody
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  • ¥5600
  • GeneTex已认证
  • 美国
  • GTX17943
  • 2025年07月16日
  • WB, ICC/IF, FACS, ChIP assay
  • Rabbit
  • Human
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    • 详细信息
    • 技术资料
    • 免疫原

      The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX17943

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, FACS, ChIP assay

    • 浓度

      Batch dependent (Please refer to the vial label for the specific concentration.)

    • 靶点

      IKK alpha (phospho Ser176/Ser180)

    • 抗体英文名

      IKK alpha (phospho Ser176/Ser180) antibody

    • 抗体名

      IKK alpha (phospho Ser176/Ser180) 抗体

    • 规格

      50 μl

    IKK alpha (phospho Ser176/Ser1

    WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody.
    The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4)
    Dilution : 1:500

    IKK alpha (phospho Ser176/Ser1

    ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-8 gene and the inactive SAT2 used as negative control target.

    IKK alpha (phospho Ser176/Ser1

    ICC/IF analysis of HeLa cells treated for 20 minutes with 50ng/ml of TNF-Alpha using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
    Fixation : 4% PFA
    Permeabilization : 0.25% Triton X-100 for 10 minutes
    Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

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