- ￥5000
- GeneTex
- 美国
- GTX11400
- 2025年07月06日
- ICC/IF, FACS
- Rat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Extracellular domain of human EGF-receptor from head and neck carcinoma.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX11400
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
ICC/IF, FACS
- 浓度：
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody, clone ICR10 binds to epitope B from EGFR
- 抗体英文名：
EGFR antibody [ICR10] (FITC)
- 抗体名：
EGFR 抗体 [ICR10] (FITC)
- 规格：
50 μg
FACS analysis of A431 cells using GTX11400 EGFR antibody [ICR10] (FITC).
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文献和实验Manuel C ScheiDmAnn et al., Cancer Res 2022 (PMID:34916221)
Donato C et al., Cell Rep 2020 (PMID:32905777)
Gkountela S et al., Cell 2019 (PMID:30633912)
Szczerba BM et al., Nature 2019 (PMID:30728496)
Vetter M et al., Breast Cancer Res 2018 (PMID:30458879)
Schwab FD et al., Microsyst Nanoeng 2022 (PMID:36561926)
min。②荧光素的准备 根据欲标记的蛋白质总量，按每毫克免疫球蛋白加0．01mg荧光色素，用分析天平准确称取所需的异硫氰酸荧光素粉末。也可用下述公式计算出免疫球蛋白、荧光素的量，还可以算出需加缓冲液的量。a．蛋白溶液：含量Amg／m1；容积Bml。b．总蛋白量(AXB)＝Crag。c．C／20～C／10＝Dmg(如蛋白含量低于20mg／ml，用C／10；如高于20mg／ml，用C／20)。d．荧光素FITC的量：(1／50～2／100)XC＝Emg。e．巳0．5mol／L(pH9．5)碳酸盐缓冲
RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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