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- 详细信息
- 技术资料
- 免疫原:
Rabbit IgG whole molecule
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Rabbit
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rabbit
- 目录编号:
GTX27090
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, Dot, ELISA, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.
- 抗体英文名:
Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP)
- 抗体名:
Goat Anti-Rabbit IgG 抗体, pre-adsorbed (HRP)
- 规格:
100 μg
GTX27090 secondary antibody was used at 1:40,000 in blocking buffer to detect a rabbit primary antibody by Western Blot. Anti p27 antibody (GTX48799, 1:1000 RT 30 minutes) showed detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose and blocked with 1% BSA-TTBS overnight at 4ºC. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
GeneTex secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein 250ng starting total load Co-incubated with GeneTex HRP conjugated Goat anti Rabbit IgG antibody (GTX27090) and Dylight 649 conjugated goat anti Rabbit IgG antiody at 1:20K in blocking buffer.
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