- 询价
- GeneTex
- 美国
- GTX04156-03
- 2025年07月12日
- IHC-P, IHC-Fr, FACS, ELISA
- Rabbit
- Sheep
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- 文献和实验
- 技术资料
- 免疫原：
Pepsin digest of Rabbit Anti-Sheep IgG(H+L).
- 亚型：
IgG F(ab')2
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Polyclonal
- 标记物：
Purified IgG
- 适应物种：
Sheep
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 目录编号：
GTX04156-03
- 级别：
Secondary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
IHC-P, IHC-Fr, FACS, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Rabbit Anti-Sheep IgG
- 抗体英文名：
Rabbit Anti-Sheep IgG antibody, F(ab')2 fragment, pre-adsorbed (AP)
- 抗体名：
Rabbit Anti-Sheep IgG 抗体, F(ab')2 fragment, pre-adsorbed (AP)
- 规格：
1 ml
ELISA analysis of purified sheep IgG using GTX04156-03 Rabbit Anti-Sheep IgG antibody, F(ab')2 fragment, pre-adsorbed (AP).
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
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